A: whether to meet the corresponding microbial limit standards of detection.
Microbial counting is used to count thermophilic bacteria and fungi that can grow under aerobic conditions. This includes plate Method, film filtration method, and Most-Probable-Number Method (MPN). In this project, the total number of aerobic bacteria, mold and yeast was determined by the pour method in the plate method. The capsule was dissolved into three different dilution levels of 1:10, 1:100 and 1:1000, and two plates were prepared in parallel according to each dilution level of detection items (total number of aerobe, total number of mold and yeast). The number of microorganisms contained in lg capsule was calculated under the corresponding conditions.
Two: whether there is a specific microorganism detection method: control bacteria inspection method. The specific bacteria detected in our products are Escherichia coli, Staphylococcus aureus and salmonella. The specific method of operation is plate marking in the plate method. Dissolve the sample with diluent to the appropriate concentration, this is the test solution; The test solution with specific (large intestine, golden yellow, samana) strains was positive; Only diluents are negative. The three reagents were cultured under specific conditions suitable for different strains and then marked on the suitable medium. Positive should show the form of a specific strain, negative should be sterile growth, the test product according to the real situation to determine whether the bacteria.
The above two points are the simplest introduction in the specific operation, and the content included is only a very simple part of the actual test items. All operations are validated. Due to the limitation of space, the verification experiments conducted in accordance with the provisions of pharmacopoeia before the specific operation are presented in the form of text arrangement.